Introduction of Pulsed Light Sterilization TechnologyⅡ
Mar. 18, 2020
The biological effects of ultraviolet wavelengths in the pulsed light spectrum have been documented. The antimicrobial effects of these wavelengths are mediated by absorption by a carbon-to-carbon double bond system that is highly bound to proteins and nucleic acids. Many researchers in this area have been extensively discussed. From one to a few pulses of flash, it has a striking antimicrobial effect. On two Petri dishes containing standard agar, instill 7 drops (each drop spreads to 1 cm2), each drop containing a series of dilutions of Staphylococcus aureus ATCC2761 and TSB cultures, one plate pulse light deal with. That is, exposure before incubation. Two 0.75J / cm2 fluence pulse flashes, that is, the total fluence is 1.5J / cm2. The other plate was not treated as a control. It was found that the concentration that could kill Staphylococcus aureus was 107CFU / cm2.
And proved that a single 0.5-l J / cm2 flash can make 105 / cm2 concentration of Listeria monocytogenes, Escherichia coli O157: H7, Bacillus pumilus and Aspergillus niger conidia ; Use 1J / cm2 to shine several times per flash, can make the above bacteria have a logarithm of 7-9 per m2.
Compared with non-pulse or continuous wave (CW) traditional UV sources, pulsed light shows a significant higher killing effect. One to several pulsed flashes can kill high-level microorganisms with high levels of exposure. It can provide a few to a dozen flashes per second, and a fraction of a second can completely sterilize. It is therefore possible to have a high material throughput. Compared with the use of a strong CWUV-C mercury gas lamp, several pulsed light flashes can kill more than 7 pairs of Aspergillus niger, while the former is not very effective, and although the exposure time is greatly increased, the sterilization effect is rarely improved or not.
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